Role of oxyradicals in the inactivation of catalase by ozone

Journal Article · Thu Dec 31 23:00:00 EST 1987 · Free Radical Biology and Medicine; (USA)

DOI:https://doi.org/10.1016/0891-5849(88)90101-3· OSTI ID:5465871

Whiteside, C; Hassan, H M ^[1]

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1. North Carolina State Univ., Raleigh (USA)

The antioxidant enzymes, catalase and superoxide dismutase, are inactivated upon exposure to ozone. In this study, the mechanism of this inactivation was examined using catalase as a model system. The data show that the inactivation of catalase is dependent on ozone concentration, time of exposure, and pH. Loss of catalase activity is accompanied with loss of the heme spectra. Tiron, desferal-Mn, trolox-c, and pyruvate protect the enzyme against ozone inactivation. SOD is less effective due to its inactivation by ozone. On the other hand, alcohols do not provide significant protection. The data suggest the possible involvement of superoxide radicals in the inactivation of catalase by ozone.

• OSTI ID: 5465871
• Journal Information: Free Radical Biology and Medicine; (USA), Journal Name: Free Radical Biology and Medicine; (USA) Vol. 5:5-6; ISSN FRBME; ISSN 0891-5849
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
BUFFERS
CATALASE
CATTLE
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
INTESTINES
MAMMALS
METABOLISM
ORGANS
OXIDOREDUCTASES
OZONE
PERFUSED ORGANS
PEROXIDASES
RADICALS
RUMINANTS
SPECTROPHOTOMETRY
SUPEROXIDE DISMUTASE
VERTEBRATES