Transport as a rate limiting step in glucose metabolism in virus-transformed cells: studies with cytochalasin B

Journal Article · Wed Dec 01 04:00:00 EST 1976 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1040890430· OSTI ID:7330256

Bissell, M J

Steady-state kinetic tracer analysis and two-dimensional chromatography and autoradiography were used to examine the relation of glucose transport to its metabolism in cultured chick embryo fibroblasts. Cytochalasin B was added to Rous sarcoma virus-infected cells to bring the rate of glucose uptake to the level of uninfected cells. It is concluded for chick cells in culture that: the transport of glucose, rather than its phosphorylation, is the rate limiting step and the difference in aerobic glycolysis between normal and virus-transformed cells at physiological glucose concentration may be a consequence of the difference in the rates of glucose uptake.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 7330256
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 89:4; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIRDS
CARBOHYDRATES
CHEMICAL REACTIONS
CHICKENS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
EMBRYOS
FIBROBLASTS
FOWL
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORYLATION
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES