Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.75.1.280· OSTI ID:7208199

Zamecnik, P C; Stephenson, M L

The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3'- and 5'-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted. The inference emerges that the tridecamer and its counterpart with blocked 3'- and 5'-hydroxyl termini enter the chick fibroblast cells, hybridize with the terminal reiterated sequences at the 3' and 5' ends of the 35S RNA, and interfere with one or more steps involved in viral production and cell transformation. Likely sites of action are (i) the circularization step of the proviral DNA intermediate, and (ii) the initiation of translation, the latter being described in the following communication (Stephenson, M. L. and Zamecnik, P. C. (1978) Proc. Natl. Acad. Sci. USA 75,285-288).

• Research Organization: Harvard Univ., Boston
• OSTI ID: 7208199
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 75:1; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

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550300 -- Cytology
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIRDS
CELL CULTURES
CHICKENS
CONNECTIVE TISSUE CELLS
DISEASES
EMBRYOS
FIBROBLASTS
FOWL
HYBRIDIZATION
INFECTIOUS DISEASES
INHIBITION
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
RNA
SOMATIC CELLS
VERTEBRATES
VIRUSES