Radioimmunoassay of lysergic acid diethylamide (LSD) in serum and urine by using antisera of different specificities

Journal Article · Tue Feb 01 00:00:00 EST 1977 · Clin. Chem.; (United States)

OSTI ID:7325925

Ratcliffe, W A ^[1]; Fletcher, S M; Moffat, A C; Ratcliffe, J G; Harland, W A; Levitt, T E

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1. Stobhill General Hospital, Glasgow

We raised high-titre antisera to two LSD-bovine serum albumin conjugates, one linked via the indole nitrogen, the other via the amide side-chain. The antisera were specific for different parts of the LSD molecule, as demonstrated by cross-reactivity studies with LSD, its metabolites, ergot alkaloids, and closely related compounds. The antisera were used to develop a double-antibody radioimmunoassay with a detection limit of about 0.4 ..mu..g of LSD per liter of unextracted urine or serum. We saw no nonspecific interference by urine, serum, or from a series of commonly used drugs. There was good correlation between immunoassay values obtained with the two antisera (r = 0.91). However, the antiserum linked via the indole nitrogen gave consistently higher results for samples from persons who had taken LSD, owing to greater cross-reactivity with LSD metabolites. Radioimmunoassay by use of two such antisera is a more specific screening procedure for LSD abuse than has been available previously. In addition, antisera cross-reacting with LSD metabolites allow measurement of these compounds, for which there is no satisfactory method at the concentrations found in biological fluids in man.

• OSTI ID: 7325925
• Journal Information: Clin. Chem.; (United States), Vol. 23:2
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
HALLUCINOGENS
RADIOIMMUNOASSAY
ANTIGEN-ANTIBODY REACTIONS
BLOOD SERUM
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MAN
TRITIUM COMPOUNDS
URINE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DRUGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
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TRACER TECHNIQUES
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550601* - Medicine- Unsealed Radionuclides in Diagnostics