Radioimmunoassays of ovine LH and ovine prolactin using polymerized second antisera

Journal Article · Sun May 15 04:00:00 EDT 1977 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(77)90622-4· OSTI ID:7110209

Chesworth, J M

A series of experiments was undertaken to assess the use of copolymerized antisera in radioimmunoassay. This paper describes assays of ovine LH and prolactin using polymerized anti-rabbit (PAR) preparations. Radioimmunoassays employing second antiserum usually require the addition of either unmodified second antiserum plus carrier serum or of DASP, sometime after the initial setting up of the tubes. In the case of DASP, agitation may then be necessary to ensure reproducibility. Polymerized antisera may be added to the tubes at the same time as the other reagents, thus removing the necessity of uncovering and rehandling them. Polymerized second antisera share with DASP the advantage that large amounts of ''carrier'' serum are not required, thus reducing the interference from this source. The use of adsorbed primary antisera as in the method of Catt et al. is as simple as that proposed here but, in my hands, has given sensitivities far below those to be expected from a double-antiserum technique.

• Research Organization: Univ. of Aberdeen, Scotland
• OSTI ID: 7110209
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 80:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LH
LTH
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
POLYMERIZATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES