A sup 125 I-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Clinical Chemistry (Winston-Salem, North Carolina); (USA)

OSTI ID:5546814

Thomson, S; Wallace, A M; Cook, B ^[1]

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1. Stobhill Hospital, Glasgow (England)

We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking ({sup 125}I)iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-({sup 125}I)iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.

• OSTI ID: 5546814
• Journal Information: Clinical Chemistry (Winston-Salem, North Carolina); (USA), Journal Name: Clinical Chemistry (Winston-Salem, North Carolina); (USA) Vol. 35:8; ISSN 0009-9147; ISSN CLCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AGE GROUPS
ANDROGENS
ANDROSTANES
ANDROSTENEDIONE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
CHILDREN
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE DISEASES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INFANTS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RESPIRATORY SYSTEM DISEASES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
UROGENITAL SYSTEM DISEASES
VERTEBRATES