Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. [UV and gamma radiation, MMS, nitrogen mustard]

Journal Article · Mon Nov 01 04:00:00 EST 1976 · Genetics; (United States)

OSTI ID:7323923

Boyd, J B; Golino, M D; Nguyen, T D; Green, M M

Thirteen X-linked mutants have been isolated in Drosophila melanogaster which render male and homozygous female larvae sensitive to the mutagen methyl methanesulfonate. Their characterization and preliminary assignment to functional groups is described. Four of these mutants are alleles of mei-41. Like previously isolated alleles of this locus, these mutants reduce fertility and increase loss and nondisjunction of the X-chromosome in homozygous females. The remaining mutants have been tentatively assigned to six functional groups (two mutants to the mus(1)101 locus, two to mus(1)102, two to mus(1)103, and one each to mus(1)104, mus(1)105, and mus(1)106. Several of the complementation groups can be distinguished on the basis of nondisjunction and cross sensitivity to mutagens. Females homozygous for the mei-41, mus(1)101 and mus(1)102 mutants exhibit elevated levels of nondisjunction. Mutants belonging to complementation groups mei-41, mus(1)101, and mus(1)104 are sensitive to nitrogen mustard (HN2) in addition to their MMS sensitivity. Among these mutants there is currently a direct correlation between sensitivity to HN2, sensitivity to 2-acetylaminofluorene and a deficiency in post-replication repair. Only the mei-41 mutants are hypersensitive to uv radiation, although several of the mutants exhibit sensitivity to ..gamma..-rays. Semidominance is observed in female larvae of the mei-41, mus(1)104, and mus(1)103 mutants after exposure to high concentrations of MMS. The properties of the mutants generally conform to a pattern which has been established for related mutants in yeast.

• Research Organization: Univ. of California, Davis
• OSTI ID: 7323923
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 84:3; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMALS
ANTIMITOTIC DRUGS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DROSOPHILA
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
FLIES
FRUIT FLIES
GAMMA RADIATION
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
METHYL METHANESULFONATE
MUTAGENS
MUTANTS
NITROGEN MUSTARD
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION