Identification of a second locus in Drosophila melanogaster required for excision repair
Journal Article
·
· Genetics; (United States)
OSTI ID:5045783
- Univ. of California, Davis
The mur(2)201 locus in Drosophila is defined by two mutant alleles that render homozygous larvae hypersensitive to mutagens. Both alleles confer strong in vivo somatic sensitivity to treatment by methyl methanesulfonate, nitrogen mustard and ultraviolet radiation but only weak hypersensitivity to X-radiation. Unlike the excision-defective mei-9 mutants identified in previous studies, the mus(2)102 mutants do not affect female fertility and do not appear to influence recombination proficiency or chromosome segregation in female meiocytes. Three independent biochemical assays reveal that cell cultures derived from embryos homozygous for the mus(2)/sup D//sup 1/ allele are devoid of detectable excision repair. 1. Such cells quantitatively retain pyrimidine dimers in their DNA for 24 hr following UV exposure. 2. No measurable unscheduled DNA synthesis is induced in mutant cultures by UV treatment. 3. Single-strand DNA breaks, which are associated with normal excision repair after treatment with either UV or N-acetoxy-N-acetyl-2-aminofluorene, are much reduced in these cultures. Mutant cells possess a normal capacity for postreplication repair and the repair of single-strand breaks induced by X-rays.
- OSTI ID:
- 5045783
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 100:2; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Excision deficiency in the mutagen-sensitive mus (2) 201/sup D1/ mutant of Drosophila
mei-9/sup a/ mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. [UV radiation, MMS, nitrogen mustard, caffeine]
Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. [UV and gamma radiation, MMS, nitrogen mustard]
Journal Article
·
Sun Dec 31 23:00:00 EST 1978
· Genetics; (United States)
·
OSTI ID:5261933
mei-9/sup a/ mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. [UV radiation, MMS, nitrogen mustard, caffeine]
Journal Article
·
Sun Oct 31 23:00:00 EST 1976
· Genetics; (United States)
·
OSTI ID:7323913
Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. [UV and gamma radiation, MMS, nitrogen mustard]
Journal Article
·
Sun Oct 31 23:00:00 EST 1976
· Genetics; (United States)
·
OSTI ID:7323923
Related Subjects
550400 -- Genetics
560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DIPTERA
DNA
DROSOPHILA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
GENES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
X RADIATION
560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DIPTERA
DNA
DROSOPHILA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
GENES
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
X RADIATION