Indomethacin induces rat uterine contractions in vitro and alters reactivity to calcium and acetylcholine

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Am. J. Obstet. Gynecol.; (United States)

DOI:https://doi.org/10.1016/0002-9378(76)90006-5· OSTI ID:7313404

Hargrove, J L; Nesbitt, D; Gaspar, M J; Ellis, L C

The initial contractions of uteri in vitro from castrated, estrogen-treated rats were markedly diminished following replacement with fresh bathing medium. Indomethacin and aspirin (10/sup -5/ to 10/sup -4/M) strongly stimulated such quiescent preparations and reduced their subsequent responsiveness to Ca/sup + +/. Reintroducing the initial bathing medium (which contained prostaglandin-like material), or adding prostaglandin F/sub 2//sub alpha/ to the fresh medium, initiated uterine contractions and restored responsiveness to calcium ion. Injections of indomethacin into castrated, estrogen-treated rats reduced initial in vitro uterine motility, abolished production of prostaglandin-like compounds, and prevented either indomethacin, aspirin, or Ca/sup + +/ from stimulating uterine contractions. Uterine responsiveness to acetylcholine in vitro was significantly reduced in rats pretreated with indomethacin.

• Research Organization: Utah State Univ., Logan
• OSTI ID: 7313404
• Journal Information: Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 124:1; ISSN AJOGA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ACETYLSALICYLIC ACID
ALKALINE EARTH METALS
AMINES
AMMONIUM COMPOUNDS
ANALGESICS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOXYLIC ACIDS
DRUGS
ELEMENTS
ESTERS
ESTROGENS
FEMALE GENITALS
HORMONES
HYDROXY ACIDS
IN VITRO
MAMMALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PHYSIOLOGY
PROSTAGLANDINS
QUATERNARY COMPOUNDS
RATS
RODENTS
STEROID HORMONES
UTERUS
VERTEBRATES