Contractility of rat testicular seminiferous tubules in vitro: prostaglandin F/sub l. cap alpha. / and indomethacin

Journal Article · Tue Jul 01 04:00:00 EDT 1975 · Prostaglandins; (United States)

OSTI ID:7292364

Buhrley, L E; Ellis, L C

The frequency of spontaneous in vitro contractions of seminiferous tubules of the rat appeared to be increased in a dose-dependent manner by prostaglandin F/sub 1 alpha/. PGF/sub 1 alpha/ treatment increased the tonus of the smooth muscle cells in the wall of the tubules as indicated by a reduction in the diameter of the tubules. When the tubules were rinsed successively with fresh Tyrode's solution, the contractile frequency was diminished. Returning the original bathing medium to the tubules restored their contractile frequency, as did treatment of the rinsed tubules with PGF/sub 1 alpha/ (10/sup -7/M). Preinjecting the rats with indomethacin tended to reduce the contractile frequency of the extirpated tubules. Treating the tubules with a solution of indomethacin for 90 min., in vitro was more effective than pretreatment in vivo in reducing contractile frequency, but a combination of these two procedures produced the greatest inhibition. PGF/sub 1 alpha/ restored the contractile frequency of the indomethacin-treated tubules. Our results indicate that PGs modulate the in vitro contractility of the tubules.

• Research Organization: Utah State Univ., Logan
• OSTI ID: 7292364
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 10:1; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANALGESICS
ANIMALS
BIOLOGICAL EFFECTS
BODY
DRUGS
GONADS
IN VITRO
MALE GENITALS
MAMMALS
ORGANS
PHYSIOLOGY
PROSTAGLANDINS
RATS
RODENTS
TESTES
VERTEBRATES