Characterization and estrogen regulation of uterine growth factor activity
Thesis/Dissertation
·
OSTI ID:7244618
Acid extracts of rat, bovine and rabbit uterus stimulated glucose transport, measured by phosphorylation of 2-deoxyglucose and DNA synthesis, measured by {sup 3}H-thymidne incorporation, in uterine tumor cells and in primary cultures of rat uterine cells. The stimulation of glucose transport was of the same magnitude and followed the same time course as estradiol stimulation in vivo. Uteri from estradiol-treated rat uteri contained 4 times more glucose transport-stimulating activity as control uteri. DNA synthetic activity in rat uterine homogenates was elevated 3-fold within 18-24 h after estradiol injection. Gel filtration showed molecular weight heterogeneity with activity eluting between 10-30 kDA. Both activities were acid and heat stable, were reduced by trypsin but not by dextran-coated charcoal. The effect of purified growth factors on DNA synthesis in primary cultures of rat uterine cells was examined. Epidermal growth factor (EGF), basic fibroblasts growth factor (bFGF), and transforming growth factor-{beta} (TGF{beta}) had no effect on {sup 3}H-thymidine incorporation.
- Research Organization:
- Texas Tech Univ., Lubbock, TX (USA)
- OSTI ID:
- 7244618
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
CARBOHYDRATES
CATTLE
CHEMICAL COMPOSITION
DNA REPLICATION
DOMESTIC ANIMALS
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
FUNCTIONS
GLUCOSE
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MITOGENS
MOLECULAR WEIGHT
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RABBITS
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
RUMINANTS
SACCHARIDES
STEROID HORMONES
STEROIDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
UTERUS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
CARBOHYDRATES
CATTLE
CHEMICAL COMPOSITION
DNA REPLICATION
DOMESTIC ANIMALS
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
FUNCTIONS
GLUCOSE
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MITOGENS
MOLECULAR WEIGHT
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RABBITS
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
RUMINANTS
SACCHARIDES
STEROID HORMONES
STEROIDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
UTERUS
VERTEBRATES