Sister chromatid exchange: the most sensitive mammalian system for determining the effects of mutagenic carcinogens
Conference
·
OSTI ID:7312035
The induction of sister chromatid exchanges in cultured cells by mutagenic carcinogens provides a sensitive cytogenetic test for the observations of the effects of these compounds on DNA. The induction of sister chromatid exchanges (SCEs), occurs at very low concentrations, far lower than those at which effects can be noticed in mammalian systems with more ordinary mutagenic and cytological tests. The SCE tests have the advantage of being very quick. For instance, CHO cells can be seeded into flasks on one day, treated on the next, and fixed 24 hours later. The cells can be quickly stained and scored. Since such a large number of SCEs is induced per chromosome, statistically significant results can be obtained when only 20 cells are scored, as contrasted to the tedious lengthy scoring required to observe significant differences with ordinary chromosome aberrations.
- Research Organization:
- California Univ., San Francisco (USA). Lab. of Radiobiology
- DOE Contract Number:
- EY-76-C-03-1012
- OSTI ID:
- 7312035
- Report Number(s):
- CONF-770559-11
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:7103966
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· Environ. Mutagen.; (United States)
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OSTI ID:6220469
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·
Tue Jun 11 00:00:00 EDT 1996
· Proceedings of the National Academy of Sciences of the United States of America
·
OSTI ID:457013
Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGY
CARCINOGENS
CELL CULTURES
CHROMATIDS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CYTOLOGY
FREQUENCY RESPONSE TESTING
GENETIC EFFECTS
IN VITRO
MUTAGENS
MUTATIONS
TESTING
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGY
CARCINOGENS
CELL CULTURES
CHROMATIDS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CYTOLOGY
FREQUENCY RESPONSE TESTING
GENETIC EFFECTS
IN VITRO
MUTAGENS
MUTATIONS
TESTING