Renal localization of /sup 99m/Tc-stannous glucoheptonate and /sup 99m/Tc-stannous dimercaptosuccinate in the rat by frozen section autoradiography. The efficiency and resolution of technetium-99m

Journal Article · Tue Mar 01 04:00:00 EST 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574659· OSTI ID:7310771

Willis, K W; Martinez, D A; Hedley-Whyte, E T; Davis, M A; Judy, P F; Treves, S

Renal localization of two /sup 99m/Tc-labeled radiopharmaceuticals, /sup 99m/Tc-dimercaptosuccinate and /sup 99m/Tc-glucoheptonate, 1 hr after injection into rats, was studied using frozen section microautoradiography and macroautoradiography (X-ray film autoradiography). The distribution of both compounds was identical within each method, but the patterns of localization between the methods were discordant. Microautoradiographs showed a well-defined and regionally specific pattern of localization in the inner renal cortex, whereas macroautoradiographs demonstrated localization throughout the cortex. In the microautoradiographs the label was found principally over the cytoplasm of proximal tubule cells. Resolution and efficiency data were determined using autoradiographs of radioactive discs. The value for resolution was 2.3 ..mu..m and that for efficiency 11 percent. The radiation dosage experienced by microscopic structures after labeling with /sup 99m/Tc-radiopharmaceuticals can be calculated from autoradiographs if the efficiency is known.

• Research Organization: Harvard Medical School, Boston
• OSTI ID: 7310771
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 69:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
DOSES
DRUGS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIATION DOSES
RADIOACTIVE MATERIALS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RATS
RODENTS
TECHNETIUM 99
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES