Mechanism of renal concentration of technetium-99m glucoheptonate

Journal Article · Fri Nov 01 04:00:00 EST 1985 · J. Nucl. Med.; (United States)

OSTI ID:6234519

Lee, H B; Blaufox, M D

Seventy female Sprague-Dawley rats were studied to determine the mechanism of tubular localization and the effects of commonly encountered changes in hydration and acid-base balance on renal uptake and urinary excretion of technetium-99m glucoheptonate ((/sup 99m/Tc)GHA). The in-vivo protein binding and protein-free plasma clearance of (/sup 99m/Tc)GHA also were quantitated. Kidney uptake of (/sup 99m/Tc)GHA averaged 11% of the injected dose in control animals. This varied slightly among groups but was significantly reduced by probenecid blockade and para-aminohippuric acid (PAH) competition to 4 and 2, respectively. Technetium-99m DMSA was not affected in its renal accumulation by these maneuvers. The total plasma clearance of (/sup 99m/Tc)GHA was lower than iodine-125( SVI)iothalamate but the clearance of the protein free supernate was higher, raising a possibility of some tubular secretion. Hepatic uptake was minimal in all groups averaging less than 1% injected dose. These data demonstrate that renal accumulation of (/sup 99m/Tc)GHA is blocked by probenecid and PAH suggesting that it is actively concentrated in the proximal tubule by enzyme systems similar to those involved in PAH and hippuran transport. It appears that (/sup 99m/Tc)GHA uptake measures a different aspect of kidney function than (/sup 99m/Tc)DMSA.

• Research Organization: Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY
• OSTI ID: 6234519
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 11; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD-PLASMA CLEARANCE
BODY
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CLEARANCE
DRUGS
ESTERS
EXCRETION
GLUCOHEPTONATE
HIPPURIC ACID
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
RODENTS
TECHNETIUM 99
TECHNETIUM ISOTOPES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES