Tc-99m glucoheptonate (GHA) renal uptake: Influence of biochemical and physiologic factors

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:7088921

Lee, H B; Blaufox, M D

Tc-99m-GHA is widely used for renal imaging but little is known about its handling by the kidney. Simultaneous single injection clearances of Tc-99m-GHA and I-125 Iothalamate were performed on 60 Sprague-Dawley female rats divided among six groups: I Control; II Dehydrated; III Mannitol infusion; IV Probenecid; V Alkaline urine (sodium bicarbonate); and VI Acid urine (ammonium chloride). Plasma concentration and urine excretion were followed during 80 minutes post injection. The livers and kidneys were removed and counted 120 minutes post injection. Total clearance of GHA was lower than Iothalamate in controls (0.90 +- 0.24 S.D. ml/min/100gr vs 1.47 +- 0.18, p < 0.005) but clearance of the protein free supernate was higher (1.67 +- 0.28 p=N.S.) raising a possibility of degree of tubular secretion. Unlike Tc-99m-Dimercaptosuccinic acid (DMSA) acidification of the urine appeared to have no effect on the amount of GHA in the urine (66.1 +- 6.35% Inj. dose vs 67.19 +- 6.05 p=n.s.) and hepatic uptake was minimal in all groups averaging less than 1%. Kidney uptake of GHA was 11.16 +- 1.53 (% Inj. dose) in controls. This varied slightly among groups but was markedly reduced by Probenecid blockade (4.08 +- 1.75, p < 0.0005). It appears that liver uptake of GHA is minimal, the non-protein bound fraction is freely filtered and its clearance correlates significantly with the GFR. Importantly renal accumulation of GHA is blocked by probenecid suggesting that it is actively concentrated in the proximal tubule by the enzyme system involved in PAH and Hippuran transport. It thus appears that measurement of renal function with GHA represents a different aspect of function than DMSA.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY
• OSTI ID: 7088921
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CARBOXYLIC ACID ESTERS
CHEMISTRY
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EXCRETION
GLANDS
GLUCOHEPTONATE
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LABELLING
LIVER
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PROCESSING
QUANTITY RATIO
RADIOISOTOPE SCANNING
RADIOISOTOPES
RATS
RENAL CLEARANCE
RODENTS
TECHNETIUM 99
TECHNETIUM ISOTOPES
UPTAKE
URINE
USES
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES