Tc-99m glucoheptonate (GHA) renal uptake: Influence of biochemical and physiologic factors
Tc-99m-GHA is widely used for renal imaging but little is known about its handling by the kidney. Simultaneous single injection clearances of Tc-99m-GHA and I-125 Iothalamate were performed on 60 Sprague-Dawley female rats divided among six groups: I Control; II Dehydrated; III Mannitol infusion; IV Probenecid; V Alkaline urine (sodium bicarbonate); and VI Acid urine (ammonium chloride). Plasma concentration and urine excretion were followed during 80 minutes post injection. The livers and kidneys were removed and counted 120 minutes post injection. Total clearance of GHA was lower than Iothalamate in controls (0.90 +- 0.24 S.D. ml/min/100gr vs 1.47 +- 0.18, p < 0.005) but clearance of the protein free supernate was higher (1.67 +- 0.28 p=N.S.) raising a possibility of degree of tubular secretion. Unlike Tc-99m-Dimercaptosuccinic acid (DMSA) acidification of the urine appeared to have no effect on the amount of GHA in the urine (66.1 +- 6.35% Inj. dose vs 67.19 +- 6.05 p=n.s.) and hepatic uptake was minimal in all groups averaging less than 1%. Kidney uptake of GHA was 11.16 +- 1.53 (% Inj. dose) in controls. This varied slightly among groups but was markedly reduced by Probenecid blockade (4.08 +- 1.75, p < 0.0005). It appears that liver uptake of GHA is minimal, the non-protein bound fraction is freely filtered and its clearance correlates significantly with the GFR. Importantly renal accumulation of GHA is blocked by probenecid suggesting that it is actively concentrated in the proximal tubule by the enzyme system involved in PAH and Hippuran transport. It thus appears that measurement of renal function with GHA represents a different aspect of function than DMSA.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY
- OSTI ID:
- 7088921
- Report Number(s):
- CONF-840619-
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
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GLUCOHEPTONATE
DIAGNOSTIC USES
LABELLING
UPTAKE
IODINE 125
KIDNEYS
RADIOISOTOPE SCANNING
RATS
TECHNETIUM 99
BIOCHEMISTRY
EXCRETION
IMAGE PROCESSING
INJECTION
LIVER
PHYSIOLOGY
QUANTITY RATIO
RENAL CLEARANCE
URINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CARBOXYLIC ACID ESTERS
CHEMISTRY
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GLANDS
HOURS LIVING RADIOISOTOPES
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROCESSING
RADIOISOTOPES
RODENTS
TECHNETIUM ISOTOPES
USES
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics