Effect of transplanted human ovarian cancer tissue on liver lipid metabolism of nude mice

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02532668· OSTI ID:7306898

Nakazawa, I ^[1]; Mead, J F

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1. Tokyo Univ.

The changes in the lipids of liver tissues of nude mice with and without transplanted human cancerous tissues were studied to clarify the effect of transplanted human tumor tissues on host liver lipid metabolism. The total lipid was extracted and separated into phospholipid, triglyceride, and other fractions by thin layer chromatography. The amounts of methyl esters of fatty acids of each lipid fraction were measured by quantitative gas liquid chromatography after each lipid fraction had been subjected to methanolysis by 5 percent HCl-methanol. The phospholipid content of liver tissues of six tumor bearing nude mice was increased and the triglyceride content decreased in comparison with these fractions in three control nude mice. The ratio of the phospholipid fatty acid content to the triglyceride fatty acid content (phospholipid:triglyceride (PL:TG)) of six tumor bearing nude mice was distributed between 7.6 and 33.5, whereas PL:TG ratios of three control nude mice were distributed between 1.7 and 3.8. This result was similar to that reported for human liver tissues of patients with malignant neoplastic disease, indicating that nude mice with transplanted human cancer may be useful for clarifying the mechanisms of the lipid-chemical changes of liver tissues of patients with malignancies.

• OSTI ID: 7306898
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 11:2; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISEASES
ESTERS
GLANDS
LIPIDS
LIVER
MAMMALS
METABOLISM
MICE
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RODENTS
SEPARATION PROCESSES
TRANSPLANTS
TRIGLYCERIDES
VERTEBRATES