Radioimmunoassay for bleomycin. [/sup 57/Co tracer technique]

Journal Article · Tue Mar 01 04:00:00 EST 1977 · J. Nucl. Med.; (United States)

OSTI ID:7304963

Elson, M K; Oken, M M; Shafer, R B

A radioimmunoassay for bleomycin, suitable for monitoring serum and urine drug levels, has been developed. Antisera were obtained from rabbits immunized with a conjugate of bleomycin and human serum albumin. Bleomycin labeled with /sup 57/Co was used as the tracer. Antibody-bound and free bleomycin were separated by precipitation with polyethylene glycol. The assay could determine bleomycin levels down to 0.025 ..mu..g/ml. The assay was precise, with a coefficient of variation of 2.8 percent. Other drugs likely to be administered in combination with bleomycin did not interfere with the radioimmunoassay.

• Research Organization: Veterans Administration Hospital, Minneapolis
• OSTI ID: 7304963
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 18:3; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBIOTICS
ANTIBODIES
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BLEOMYCIN
COBALT 57
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
IMMUNE REACTIONS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES