Improvement of specificity in radioimmunoassays
Journal Article
·
· Clin. Chim. Acta, v. 44, no. 3, pp. 333-340
The specificity of a radioimmunoassay for serum digoxin was investigated. The assay used /sup 3/H -digoxin and rabbit antiserum to a digoxin-albumin conjugate, with separation of antibody-bound and free tracer by adsorption of the latter on charcoal. By exploiting the kinetics of dissociation of the steroid antibody complex, digoxin could be distinguished from the other steroids. Comparison of apparent digoxin levels, measured in the two types of assay, based respectively on the principles of simultaneous addition and preincubation, imposes a stringent specificity test in the assay of serum samples. The double assay principle is suggested for consideratton in all radioimmunoassays utilizing incompletely specific antisera. (INIS)
- Research Organization:
- Home Office Central Research Establishment, Aldermaston, Eng.
- NSA Number:
- NSA-29-008162
- OSTI ID:
- 4412110
- Journal Information:
- Clin. Chim. Acta, v. 44, no. 3, pp. 333-340, Journal Name: Clin. Chim. Acta, v. 44, no. 3, pp. 333-340; ISSN CCATA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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