Dose-response and time-response study of erythrocytosis in rats after intrarenal injection of nickel subsulfide

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Ann. Clin. Lab. Sci.; (United States)

OSTI ID:7287113

Morse, E E; Lee, T Y; Reiss, R F; Sunderman, Jr, F W

Erythrocytosis was induced in Fischer rats by intrarenal (ir) injection of nickel subsulfide (Ni/sub 3/S/sub 2/) in dosages ranging from 0.6 to 10 mg per rat. Measurements of blood packed cell volume (PCV) became increased by one month after ir injection of Ni/sub 3/S/sub 2/; reached maximum values at approximately two months; and gradually returned to control values by seven months. The duration and magnitude of erythrocytosis were related to the dosage of Ni/sub 3/S/sub 2/. Increases in hemoglobin concentrations and erythrocyte counts (RBC) in Ni/sub 3/S/sub 2/-treated rats were consistently proportional to blood PCV values. Blood hemoglobin concentration was 24.9 +- 1.2 g per dl at two months after bilateral ir injection of Ni/sub 3/S/sub 2/ (10 mg per rat), versus 15.8 +- 0.5 g per dl in saline-injected controls (P less than 0.001). No significant changes occurred in leukocyte or platelet counts of Ni/sub 3/S/sub 2/-treated rats. Autopsy of rats killed two months after ir injection of Ni/sub 3/S/sub 2/ showed marked erythroid hyperplasia of bone marrow and fibrotic needle tracts in renal parenchyma with localized deposits of particles of Ni/sub 3/S/sub 2/. In contrast to the erythrocytosis induced by ir injection of Ni/sub 3/S/sub 2/, administration of Ni/sub 3/S/sub 2/ by intramuscular (im) injection (10 mg per rat) had no significant effect upon blood PCV, RBC or hemoglobin values or upon morphology of bone marrow.

• Research Organization: Univ. of Connecticut School of Medicine, Farmington
• OSTI ID: 7287113
• Journal Information: Ann. Clin. Lab. Sci.; (United States), Journal Name: Ann. Clin. Lab. Sci.; (United States) Vol. 7:1; ISSN ACLSC
• Country of Publication: United States
• Language: English

Related Subjects

550000* -- Biomedical Sciences
Basic Studies
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BLOOD PLATELETS
BODY
BODY FLUIDS
BONE MARROW
CARBOXYLIC ACIDS
CHALCOGENIDES
DOSE-RESPONSE RELATIONSHIPS
ERYTHROCYTES
GLOBIN
HEMATOPOIETIC SYSTEM
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INJECTION
INTAKE
KIDNEYS
LEUKOCYTES
MAMMALS
NICKEL COMPOUNDS
NICKEL SULFIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
PORPHYRINS
PROTEINS
RATS
RODENTS
SULFIDES
SULFUR COMPOUNDS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
VOLUME