Cooperation of nonsyngeneic tolerant lymphocytes: genetic restriction. [X Radiation]
We have used a previously devised in vivo experimental model to investigate the ability of mouse thymus-dependent (T) and bone marrow-derived (B) lymphocytes to cooperate in immune (humoral) rejection of rat Yoshida ascites sarcoma (YAS). Because of conflicting reports in the literature concerning the effectiveness of T--B cooperation across major histocompatibility complex (MHC) barriers, we explored the interaction of T and B lymphocytes from mutually tolerant animals. Tolerance was achieved by establishing radiation chimeras of B6 ..-->.. B6D2F/sub 1/ and D2 ..-->.. B6D2F/sub 1/ constitutions. Chimeras' erythrocytes and spleen cells were shown by serological analysis to be the donor type. When the chimera was to serve as the tumor host or B-cell source, it was thymectomized prior to irradiation and reconstitution (TIR). Tolerance was evaluated by noting the inability of chimeric spleen cells to effect graft-versus-host damage upon injection into TIR host-type mice and the markedly reduced anti-host-type reactivity in short-term (/sup 3/H)thymidine-uptake tests. Successful cooperation, manifested by YAS rejection, was seen whenever donor T and host B lymphocytes were syngeneic. Parental (P) T cells enabled F/sub 1/ TIR mice to reject YAS, but the reciprocal was not true: F/sub 1/ donor T cells did not cooperate with B cells in parental TIR mice. However, when the host B lymphocytes were tolerant P cells, i.e., in a P ..-->.. F/sub 1/ TIR chimera, injected F/sub 1/ T lymphocytes did cooperate successfully. The final test of allogeneic T and B cells gave the clear-cut negative answer that, even when tolerant mice are used as sources of lymphocytes, cooperation does not occur. These results therefore confirm that T and B lymphocytes must at least share one MHC haplotype in order to cooperate.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 7285150
- Journal Information:
- Cell. Immunol.; (United States), Vol. 33:1
- Country of Publication:
- United States
- Language:
- English
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IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
LYMPHOCYTES
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MICE
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SARCOMAS
SPLEEN CELLS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
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560152* - Radiation Effects on Animals- Animals