Cooperation of nonsyngeneic tolerant lymphocytes: genetic restriction. [X Radiation]

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(77)90135-6· OSTI ID:7285150

Marusic, M; Goodman, J W; Shinpock, S G

We have used a previously devised in vivo experimental model to investigate the ability of mouse thymus-dependent (T) and bone marrow-derived (B) lymphocytes to cooperate in immune (humoral) rejection of rat Yoshida ascites sarcoma (YAS). Because of conflicting reports in the literature concerning the effectiveness of T--B cooperation across major histocompatibility complex (MHC) barriers, we explored the interaction of T and B lymphocytes from mutually tolerant animals. Tolerance was achieved by establishing radiation chimeras of B6 ..-->.. B6D2F/sub 1/ and D2 ..-->.. B6D2F/sub 1/ constitutions. Chimeras' erythrocytes and spleen cells were shown by serological analysis to be the donor type. When the chimera was to serve as the tumor host or B-cell source, it was thymectomized prior to irradiation and reconstitution (TIR). Tolerance was evaluated by noting the inability of chimeric spleen cells to effect graft-versus-host damage upon injection into TIR host-type mice and the markedly reduced anti-host-type reactivity in short-term (/sup 3/H)thymidine-uptake tests. Successful cooperation, manifested by YAS rejection, was seen whenever donor T and host B lymphocytes were syngeneic. Parental (P) T cells enabled F/sub 1/ TIR mice to reject YAS, but the reciprocal was not true: F/sub 1/ donor T cells did not cooperate with B cells in parental TIR mice. However, when the host B lymphocytes were tolerant P cells, i.e., in a P ..-->.. F/sub 1/ TIR chimera, injected F/sub 1/ T lymphocytes did cooperate successfully. The final test of allogeneic T and B cells gave the clear-cut negative answer that, even when tolerant mice are used as sources of lymphocytes, cooperation does not occur. These results therefore confirm that T and B lymphocytes must at least share one MHC haplotype in order to cooperate.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7285150
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 33:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MICE
MOSAICISM
NEOPLASMS
RADIATION CHIMERAS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SARCOMAS
SOMATIC CELLS
SPLEEN CELLS
THYMOCYTES
VERTEBRATES
X RADIATION