In vivo cooperation of murine lymphocytes sharing one haplotype of the transplantation genes: a requirement for tolerance of the nonshared haplotype
The ability of mouse thymus-dependent (T) and bone marrow-derived (B) lymphocytes to cooperate in the (humoral) rejection of the rat Yoshida ascites sarcoma (YAS) was investigated. Mice of two hybrid constitutions that had one parent of the same strain and the other of a different strain were used. T and B cells thus shared one haplotype of the transplantation genes, but the other haplotype was different so that allogeneic recognition could take place in both directions. To investigate the influence of allogeneic recognition on T-B cell cooperation mice were also made mutually tolerant by establishing long-term radiation chimeras. Erythrocytes and spleen cells of the chimera were shown by serologic analysis to be donor-type. When the chimera was to serve as tumor host and B cell source, it was thymectomized before irradiation and reconstitution with Thy 1.2 antisera and complement-treated bone marrow (TIR). The evidence of tolerance was markedly reduced antihost-type reactivity in short-term in vivo (/sup 3/H)thymidine uptake studies. Successful cooperation, manifested by YAS rejection in TIR mice given splenic T cells, was seen (a) whenever the transferred T cells and the cells of the TIR recipient were syngeneic, and (b) when the T cells were nonsyngeneic with the cells in TIR recipients but were obtained from chimeric donors and injected into chimeric recipients. Chimerism of only T cell donors or TIR recipients was not sufficient for YAS rejection to be induced.The data suggest that the allogeneic recognition on either T or B lymphocytes may thwart otherwise successful cell cooperation.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5372883
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 122:3; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODY FORMATION
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DISEASES
EXPERIMENTAL NEOPLASMS
GENES
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IMMUNE REACTIONS
IMMUNITY
IN VIVO
ISOTOPES
LABELLING
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOSAICISM
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION CHIMERAS
RADIOISOTOPES
RIBOSIDES
RODENTS
SARCOMAS
SOMATIC CELLS
THYMIDINE
TOLERANCE
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES