In vivo cooperation of murine lymphocytes sharing one haplotype of the transplantation genes: a requirement for tolerance of the nonshared haplotype
The ability of mouse thymus-dependent (T) and bone marrow-derived (B) lymphocytes to cooperate in the (humoral) rejection of the rat Yoshida ascites sarcoma (YAS) was investigated. Mice of two hybrid constitutions that had one parent of the same strain and the other of a different strain were used. T and B cells thus shared one haplotype of the transplantation genes, but the other haplotype was different so that allogeneic recognition could take place in both directions. To investigate the influence of allogeneic recognition on T-B cell cooperation, mice were also made mutually tolerant by establishing long-term radiation chimeras. Erythrocytes and spleen cells of the chimera were shown by serologic analysis to be donor-type. When the chimera was to serve as tumor host and B cell source, it was thymectomized before irradiation and reconstitution with Thy 1.2 antisera and complement-treated bone marrow (TIR). The evidence of tolerance was markedly reduced anti-host-type reactivity in short-term in vivo (/sup 3/H)thymidine uptake studies. Successful cooperation, manifested by YAS rejection in TIR mice given splenic T cells, was seen (a) whenever the transferred T cells and the cells of the TIR recipient were syngeneic, and (b) when the T cells were nonsyngeneic with the cells in TIR recipients but were obtained from chimeric donors and injected into chimeric recipients. Chimerism of only T cell donorsor TIR recipients was not sufficient for YAS rejection to be induced. The data suggest that the allogeneic recognition on either T or B lymphocytes may thwart otherwise successful cell cooperation.
- Research Organization:
- Univ. of Tennessee Graduate School of Biomedical Sciences, Oak Ridge
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5423359
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 122:3; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ASCITES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
HEMATOPOIETIC SYSTEM
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IN VIVO
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOSAICISM
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PYRIMIDINES
RADIATION CHIMERAS
RIBOSIDES
RODENTS
SARCOMAS
SOMATIC CELLS
SYMPTOMS
THYMIDINE
THYMOCYTES
THYMUS
TISSUES
TRANSPLANTS
TRITIUM COMPOUNDS
VERTEBRATES