Radiation-induced DNA damage and lethality in E. coli as modified by the antitumor agent cis-dichlorodiammineplatinum(II)

Journal Article · Mon Aug 01 04:00:00 EDT 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574686· OSTI ID:7282263

Richmond, R C ^[1]; Zimbrick, J D; Hykes, D L

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1. Army Natick Development Center, MA

The antitumor drug, cis-Pt(NH/sub 3/)/sub 2/Cl/sub 2/, has been tested on stationary phase cells of E. coli C for toxicity, radiation sensitization, and effect on radiation-induced thymine base residue release from cellular DNA. Sensitization in N/sub 2/-saturated suspensions is about 60% greater than that obtained in O/sub 2/-saturated suspensions. Additionally, a small component of low-dose radiation sensitivity is observed for bacteria irradiated in the presence of sensitizing concentrations of the Pt complex under anoxic, but not oxic, conditions. When stationary phase cells which contain /sup 3/H-thymine-labeled DNA are irradiated and treated under conditions designed to inhibit enzyme action, various tritiated products are obtained from the cellular DNA. The product of highest yield has been identified as thymine, and more thymine is produced when oxygen is present during irradiation than when it is absent. When cells are irradiated in the presence of a sensitizing concentrations of the platinum drug, the thymine yield is reduced and shows little difference between oxic and anoxic conditions.

• OSTI ID: 7282263
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CRYOGENIC FLUIDS
DISEASES
DNA
DRUGS
ELEMENTS
ESCHERICHIA COLI
FLUIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INJURIES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NONMETALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PLATINUM COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RESPONSE MODIFYING FACTORS
THYMINE
TOXICITY
TRANSITION ELEMENT COMPOUNDS
TRITIUM
URACILS
YEARS LIVING RADIOISOTOPES