Double labeling with (/sup 3/H)thymidine and (/sup 125/I)iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo. [Mice]

Journal Article · Wed Jan 01 04:00:00 EST 1975 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.67.2.484· OSTI ID:7279297

Myers, D K; Feinendegen, L E

Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both (/sup 3/H)thymidine (TdR) and (/sup 125/I)iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of (/sup 3/H)TdR/(/sup 125/I)IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day after injection, but survival of the DNA from whole cells could be demonstrated in some cases.

• Research Organization: Kernforschungsanlage, Juelich, Ger.
• OSTI ID: 7279297
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 67; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DNA
DOUBLE LABELLING
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROLYSIS
HYDROXY COMPOUNDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
INTRAVENOUS INJECTION
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
LYSIS
MAMMALS
MICE
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RIBOSIDES
RODENTS
SOLVOLYSIS
SOMATIC CELLS
THYMIDINE
THYMOCYTES
TRACER TECHNIQUES
TRITIUM
URACILS
VERTEBRATES
YEARS LIVING RADIOISOTOPES