Effect of radiation on normal hematopoiesis and on viral induced cancers of the hematopoietic system. Technical progress report, August 1, 1974--May 1, 1975. [Mice, x radiation]

Technical Report · Wed Jan 01 04:00:00 EST 1975

DOI:https://doi.org/10.2172/7270254· OSTI ID:7270254

Okunewick, J. P.

Studies carried out during the above period on viral leukemia have conclusively shown that the pluripotent hematopoietic colony forming stem cell (CFU-S) is a target cell for the leukemia virus. Treatment of this cell population with antiserum prepared in syngeneic mice against the disease resulted in inactivation of up to 50 percent of the CFU-S obtained from the spleens of viral leukemic mice. At the same time, normal serum had no effect on these cells, nor did the antiserum have any effect on normal CFU-S. Data indicated that a considerable time delay, on the order of a week, preceded the expression of the viral antigen in the leukemic CFU-S, but that it could be seen at all times after that up to the terminal point of the disease. We examined the effect of the virus on DNA synthesis (S-phase cells) in the CFU-S immediately after virus injection. The results showed that a doubling of the number of cells in S could be seen as early as four hours after introduction of the virus into the animal. Studies with ethidium bromide, an inhibitor of viral reverse transcriptase, were found to be in agreement with this observation. When given to viral leukemic animals in combination with fractionated exposure to x-ray, the data suggested that ethidium bromide did act to extend survival somewhat, but not much over that seen through the use of x-ray alone.

• Research Organization: Allegheny General Hospital, Pittsburgh, Pa. (USA). Cancer Research Unit
• OSTI ID: 7270254
• Report Number(s): COO-3097-11
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BONE MARROW
BROMIDES
BROMINE COMPOUNDS
CELL CYCLE
CFU
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
HALIDES
HALOGEN COMPOUNDS
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IMMUNE SERUMS
IONIZING RADIATIONS
IRRADIATION
LEUKEMIA
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
SYNTHESIS
TISSUES
VERTEBRATES
VIRUSES
X RADIATION