Intracellular transport of pancreatic zymogens during caerulein supramaximal stimulation

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:7266795

Saito, I; Hashmimoto, S; Saluja, A; Steer, M L; Meldolesi, J ^[1]

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1. Univ. of Milan (Italy) Beth Israel Hospital, Boston, MA (USA)

Rats infused with a dose of the secretagogue caerulein that is in excess of that which stimulates a maximal rate of pancreatic digestive enzyme secretion develop acute edematous pancreatitis. The authors have previously noted that infusion of this dose of caerulein induces the appearance of large heterogeneous vacuoles in acinar cell, blockage of exocytosis, and intracellular accumulation of digestive zymogens. The current studies were performed to further elucidate these phenomena at the electron microscopic level of resolution and employed the techniques of pulse labeling, radioautography, and immunolocalization. Rats were infused with caerulein for 1 h, given a pulse of ({sup 3}H)phenylalanine, and killed at selected times during the subsequent 5- to 180-min postpulse period during which caerulein infusion was continued. Transport from the endoplasmic reticulum to the Golgi cisternae was not altered by supramaximal stimulation, but transport through post-Golgi elements was altered. In particular, the maturation of condensing vacuoles into zymogen granules was found to be impaired. Thus these studies indicate that the large heterogeneous vacuoles that appear during supramaximal secretagogue stimulation and that contain admixed digestive zymogens and lysosomal hydrolases arise by at least two mechanisms, impaired condensing vacuole maturation and crinophagy.

• OSTI ID: 7266795
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:4; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AUTORADIOGRAPHY
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
ENDOPLASMIC RETICULUM
ENZYMES
GLANDS
HYDROGEN COMPOUNDS
HYDROLASES
LYSOSOMES
MAMMALS
MEMBRANE TRANSPORT
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PANCREAS
PHENYLALANINE
RATS
RODENTS
SECRETION
SUBCELLULAR DISTRIBUTION
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES