Identification of SNAREs that mediate zymogen granule exocytosis

Journal Article · Fri Aug 03 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.05.128· OSTI ID:20991469

Pickett, James A ^[1]; Campos-Toimil, Manuel ^[2]; Thomas, Paul ^[3]; Edwardson, J Michael ^[1]

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1. Department of Pharmacology, Tennis Court Road, University of Cambridge, Cambridge CB2 1PD (United Kingdom)
2. Department of Pharmacology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela (Spain)
3. Henry Wellcome Laboratory for Cell Imaging, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ (United Kingdom)

A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. We attempted to identify the SNAREs involved in zymogen granule exocytosis. Antibodies against syntaxins 2 and 3, SNAP-23 and VAMP 8, and the corresponding recombinant SNAREs, inhibited amylase secretion from streptolysin O-permeabilised acini; other anti-SNARE antibodies and SNAREs had no effect. Botulinum neurotoxin C, which cleaved syntaxin 2 and (to a lesser extent) syntaxin 3, but not syntaxins 4, 7 or 8, also inhibited exocytosis. We propose that syntaxin 2, SNAP-23 and VAMP 8 mediate primary granule-plasma membrane fusion. Syntaxin 3 may be involved in secondary granule-granule fusion.

• OSTI ID: 20991469
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 359; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AMYLASE
ANTIBODIES
CELL MEMBRANES
PANCREAS
SECRETION