Transplantable pancreatic acinar carcinoma

Journal Article · Sat Mar 14 23:00:00 EST 1981 · Cancer (Philadelphia); (United States)

DOI:https://doi.org/10.1002/1097-0142(19810315)47:6+<1535::AID-CNCR2820471416>3.0.CO;2-1· OSTI ID:5443383

Warren, J R; Reddy, J K

Fragments of the nafenopin-induced pancreatic acinar cell carcinoma of rat have been examined in vitro for patterns of intracellular protein transport and carbamylcholine-induced protein discharge. Continuous incubation of the fragments with (3H)-leucine for 60 minutes resulted in labeling of rough endoplasmic reticulum, Golgi cisternae, and mature zymogen granules, revealed by electron microscope autoradiography. This result indicates transport of newly synthesized protein from the rough endoplasmic reticulum to mature zymogen granules in approximately 60 minutes. The secretagogue carbamylcholine induced the discharge of radioactive protein by carcinoma fragments pulse-chase labeled with (3H)-leucine. A maximal effective carbamylcholine concentration of 10(-5) M was determined. The acinar carcinoma resembles normal exocrine pancreas in the observed rate of intracellular protein transport and effective secretagogue concentration. However, the acinar carcinoma fragments demonstrated an apparent low rate of carbamylcholine-induced radioactive protein discharge as compared with normal pancreatic lobules or acinar cells. It is suggested that the apparent low rate of radioactive protein discharge reflects functional immaturity of the acinar carcinoma. Possible relationships of functional differentiation to the heterogeneous cytodifferentiation of the pancreatic acinar carcinoma are discussed.

• OSTI ID: 5443383
• Journal Information: Cancer (Philadelphia); (United States), Journal Name: Cancer (Philadelphia); (United States) Vol. 47:6 Suppl; ISSN CANCA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BIOLOGY
BODY
CARBOXYLIC ACIDS
CARCINOMAS
CELL CONSTITUENTS
CHEMISTRY
CYTOLOGY
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE GLANDS
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PERMEABILITY
PROTEINS
RATS
RODENTS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES