Radioimmunoassay measurement of secretin half-life in man

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Gastroenterology; (United States)

OSTI ID:7256551

Kolts, B E; McGuigan, J E

High titer antibodies to secretin were produced in rabbits by immunization with purified porcine secretin (from GIH) conjugated to bovine serum albumin in complete Freund's adjuvant. Synthetic porcine secretin (from Squibb) was radiolabeled with /sup 125/I using chloramine-T and radiosecretin was purified using talc-silica and fibrocellulose powder. Specificity of antibodies to secretin was extensively evaluated in studies examining (1) cross-reactivity with other naturally occurring hormones, (2) parallelism of serum dilutions and secretin standards, (3) quantitative recovery of secretin added to serum samples, and (4) changes in circulating immunoreactive secretin in response to intraduodenal acidification. The mean fasting serum secretin concentration in 10 dogs was 65 +- 5 (SEM) pg per ml and in 21 healthy human volunteers it was 69 +- 5 pg per ml. In response to intraduodenal instillation of 10 mEq of HCl, portal venous secretin concentrations increased from a mean fasting value of 52 to 450 pg per ml within 2 min; peripheral venous secretin increased from 66 to 229 pg per ml within 5 min. No significant changes in peripheral serum immunoreactive secretin concentrations were identified in dogs after a protein meal. Intravenously administered secretin in man disappeared from the circulation with mean half-life 4.06 +- 0.82 (SEM) min.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 7256551
• Journal Information: Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 72:1; ISSN GASTA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SECRETIN
TRACER TECHNIQUES
VERTEBRATES