Development of radioimmunoassay for motilin

Journal Article · Sat Jul 01 04:00:00 EDT 1978 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(78)90684-X· OSTI ID:6740840

Tai, H H; Chey, W Y

A highly specific and sensitive radioimmunoassay for motilin has been developed. High titers of motilin antisera were produced in rabbits by repeated immunization with synthetic porcine motilin coupled to bovine serum albumin. Motilin was radioiodinated with Na(/sup 125/I) by the chloramine-T method and was purified using a CM-Sephadex C-25 column eluted with ammonium acetate gradient at pH 5.9. A specific radioactivity of 300 to 400 ..mu..Ci/..mu..g was usually obtained. Untreated plasma showed significant nonspecific interference in the assay; however, the interference could be minimized by extracting the plasma with methanol. All of the known gastrointestinal hormones were tested and showed no cross-reactivity at 10 ng/ml. Plasma motilin levels in dogs were significantly increased within 3 min, after an intraduodenal bolus injection of 50 ml of 0.1 N HCl. This increase coincided with a marked increase in myoelectrical activities of the duodenum.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6740840
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 87:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SENSITIVITY
TRACER TECHNIQUES
VERTEBRATES