Use of a macrocyclic antibiotic as the chiral selector for enantiomeric separations by TLC
- Univ. of Missouri, Rolla, MO (United States). Dept. of Chemistry
The macrocyclic antibiotic, vancomycin, was used as a chiral mobile phase additive for the thin layer chromatographic (TLC) resolution of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatized amino acids, racemic drugs and dansyl-amino acids. Excellent separations were achieved for most of these compounds in the reversed phase mode. Both the nature of the stationary phase and the composition of the mobile phase strongly influenced enantiomeric resolution. The best results were obtained using diphenyl stationary phases. Acetonitrile was the organic modifier that produced the most effective separations with the shortest development times. It is highly likely that macrocyclic antibiotics will play a major role in future enantiomeric separations.
- DOE Contract Number:
- FG02-88ER13819
- OSTI ID:
- 7254111
- Journal Information:
- Journal of Liquid Chromatography; (United States), Vol. 17:8; ISSN 0148-3919
- Country of Publication:
- United States
- Language:
- English
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