Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: effect of butylated hydroxytoluene and cold shock
Journal Article
·
· Biol. Reprod.; (United States)
Spin label probes were used in conjunction with measurements of metabolic rate and electron microscopy to characterize bovine sperm membranes. Aqueous compartments, membrane hydrocarbon zones and lipid : water interfaces were studied separately using appropriate spin labels. For sperm suspended in aqueous medium, the cold shock associated with rapid cooling from room temperature to 0/sup 0/ increased membrane permeability. This membrane damage was readily detected using spin labels but was not detected using thin section electron microscopy. This change was prevented by the addition of butylated hydroxy toluene (BHT). BHT provided partial protection against further damage caused by freezing sperm on solid CO/sub 2/. ESR techniques provide a rapid means to quantify the changes in sperm membranes occurring during the epididymal maturation of sperm and subsequent events within the female tract leading to fertilization. The technique also could be used to assess damage to sperm, ova or embryos during preparation for storage in cryoprotective diluents.
- Research Organization:
- Pennsylvania State Univ., University Park
- OSTI ID:
- 7248396
- Journal Information:
- Biol. Reprod.; (United States), Journal Name: Biol. Reprod.; (United States) Vol. 14:4; ISSN BIREB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Use of spin labels to evaluate effects of cold shock and osmolality on sperm
Radiosensitization of Drosophila sperm by commonly used food additives. Butylated hydroxyanisole and butylated hydroxytoluene
Inactivation of the lipid-containing bacteriophage PM2 by butylated hydroxytoluene
Journal Article
·
Mon May 01 00:00:00 EDT 1978
· Biol. Reprod.; (United States)
·
OSTI ID:6687208
Radiosensitization of Drosophila sperm by commonly used food additives. Butylated hydroxyanisole and butylated hydroxytoluene
Journal Article
·
Mon Dec 31 23:00:00 EST 1973
· Int. J. Radiat. Biol., v. 25, no. 1, pp. 67-72
·
OSTI ID:4331428
Inactivation of the lipid-containing bacteriophage PM2 by butylated hydroxytoluene
Journal Article
·
Sun Nov 30 23:00:00 EST 1975
· Antimicrob. Agents Chemother. (1961-70); (United States)
·
OSTI ID:7234127
Related Subjects
550300 -- Cytology
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUEOUS SOLUTIONS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL SHOCK
CATTLE
CRESOLS
DISEASES
DISPERSIONS
DOMESTIC ANIMALS
ELECTRON MICROSCOPY
ELECTRON SPECTROSCOPY
ELECTRON SPIN RESONANCE
FERTILITY
FREEZING
GAMETES
GERM CELLS
HYDROCARBONS
HYDROXY COMPOUNDS
INTERFACES
LABELLING
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
METABOLISM
MICROSCOPY
MIXTURES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PERMEABILITY
PHENOLS
RESONANCE
RUMINANTS
SOLUTIONS
SPECTROSCOPY
SPERMATOZOA
TEMPERATURE EFFECTS
VERTEBRATES
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUEOUS SOLUTIONS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL SHOCK
CATTLE
CRESOLS
DISEASES
DISPERSIONS
DOMESTIC ANIMALS
ELECTRON MICROSCOPY
ELECTRON SPECTROSCOPY
ELECTRON SPIN RESONANCE
FERTILITY
FREEZING
GAMETES
GERM CELLS
HYDROCARBONS
HYDROXY COMPOUNDS
INTERFACES
LABELLING
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
METABOLISM
MICROSCOPY
MIXTURES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PERMEABILITY
PHENOLS
RESONANCE
RUMINANTS
SOLUTIONS
SPECTROSCOPY
SPERMATOZOA
TEMPERATURE EFFECTS
VERTEBRATES