Inactivation of the lipid-containing bacteriophage PM2 by butylated hydroxytoluene
Several factors have been investigated which are of significance in the inactivation of PM2, a lipid-containing bacterial virus, by butylated hydroxytoluene (BHT). Studies of the time dependence of inactivation during exposure to BHT showed that virus killing occurs rapidly, with the majority of the effect taking place in the first 5 min. The degree of inactivation is dependent upon the initial virus titer, the solvent from which BHT is added, and the presence of a variety of protective agents, including surfactants, bovine serum albumin, and bacterial cells. Sucrose gradient analysis of /sup 32/P-labeled, BHT-treated virus was used to determine the degree to which the virion is disrupted by BHT. These experiments show that the /sup 32/P-labeled molecules are converted into very slowly sedimentable material by BHT treatment, indicating complete destruction of the virus particle.
- Research Organization:
- Pennsylvania State Univ., University Park
- OSTI ID:
- 7234127
- Journal Information:
- Antimicrob. Agents Chemother. (1961-70); (United States), Journal Name: Antimicrob. Agents Chemother. (1961-70); (United States) Vol. 8:6; ISSN AACHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Butylated hydroxytoluene inactivates lipid-containing viruses
Enhancement of allergic responses in vivo and in vitro by butylated hydroxytoluene