Excess membrane cholesterol alters smooth muscle cell and endothelial cell function

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7245989

Bialecki, R A

The object of this study was to determine the effects of cholesterol, as an independent variable, on arterial function and to clarify the cellular mechanism underlying these alterations. For these experiments, rabbit carotid arteries were perfused in vitro with medium-bicarbonate buffer. For cholesterol enrichment, this buffer was supplemented with serum and cholesterol-rich liposomes. Following perfusion, ring segments of the arteries were evaluated isometrically for alternations in maximal stress development, vasoconstrictor sensitivities, relaxation responses as well as FC and PL contents. In parallel studies, vascular rings were tested for alterations in basal and agonist-activated calcium influx. The mechanism of cholesterol-induced alterations in arterial smooth muscle cell function was examined by determining the effects of cholesterol on {sup 45}Ca influx under basal and agonist-activated conditions. Cholesterol-induced alterations in arterial vasodilator responses were investigated by assessing changes in endothelium-dependent and endothelium-independent relaxation responses.

• Research Organization: Medical Coll. of Pennsylvania, Philadelphia, PA (USA)
• OSTI ID: 7245989
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BLOOD VESSELS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CAROTID ARTERIES
CELL CONSTITUENTS
CELL MEMBRANES
CHOLESTEROL
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENDOTHELIUM
EVEN-ODD NUCLEI
FUNCTIONS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIPOSOMES
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MUSCLES
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PERFUSED TISSUES
RABBITS
RADIOISOTOPES
STEROIDS
STEROLS
TISSUES
TRACER TECHNIQUES
VASOCONSTRICTION
VASODILATORS
VERTEBRATES