Correction of enhanced endothelial permeability by cessation of cholesterol feeding

Journal Article · Sun Feb 01 04:00:00 EST 1987 · J. Vasc. Surg.; (United States)

DOI:https://doi.org/10.1067/mva.1987.avs0050336· OSTI ID:6859388

Menzoian, J O; Haudenschild, C C; Shipman, J L; Nickerson, C J; Fuller, R M; Chobanian, A V

Changes in endothelial permeability and the transport of macromolecules may be important in the initiation and/or progression of atherosclerosis. We have previously shown, with a carotid artery preparation isolated in situ with intact adventitia, that long-term cholesterol feeding in rabbits will result in a seven- to tenfold increase in /sup 125/I albumin transport across the artery into the systemic circulation. The current studies were undertaken to determine whether this abnormality of enhanced permeability could be reversed by cessation of cholesterol feeding and correction of the hyperlipidemia. Two groups of rabbits were fed either a standard Rabbit Chow or a diet containing 1.5% cholesterol and 5.2% corn oil for 12 to 15 weeks. Another group of rabbits was given cholesterol for 12 to 15 weeks with change to standard rabbit chow for an additional 22 to 24 weeks after which albumin transport studies were then performed. Mean plasma cholesterol level after 12 to 15 weeks of cholesterol feeding was 2052 +/- 395 mg/dl. After the animals were withdrawn from the cholesterol diet for 22 to 24 weeks, the mean plasma cholesterol level decreased to 80 +/- 21 mg/dl. The mean plasma cholesterol value in chow-fed animals was 39 +/- 6 mg/dl. Perfusion studies were done with /sup 125/I-labeled albumin and plasma radioactivity served as a measure of transport across the carotid artery. The average level of albumin transport across the artery into venous blood in the cholesterol-fed animals (13,911 dpm/ml of plasma) was significantly greater than that of control animals (2049 dpm/ml of plasma).

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 6859388
• Journal Information: J. Vasc. Surg.; (United States), Journal Name: J. Vasc. Surg.; (United States) Vol. 2; ISSN JVSUE
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
ANIMALS
ARTERIES
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CAROTID ARTERIES
CHOLESTEROL
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PERFUSED TISSUES
PERMEABILITY
PROTEINS
RABBITS
RADIOISOTOPES
STEROIDS
STEROLS
TISSUES
VASCULAR DISEASES
VERTEBRATES