Free radicals release vasodilator(s) from endothelial and smooth muscle cells

Conference · Thu Mar 14 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5238563

Chen, X; Hu, Y C; Gillis, C N ^[1]

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1. Yale Univ., New Haven, CT (United States)

Electrolysis-generated free radicals (ES-FR) injure the endothelium (EC) of rabbit lungs perfused in situ. The authors now report that ES-FR release EDRF-like substance(s) from both vascular EC and smooth muscle sources. Rabbit aortic segments with or without endothelium or columns of bovine aortic EC grown on biosilon beads were perfused with Krebs solution containing 6 uM indomethacin and 10 units/ml SOD. Effluent passed over two endothelial-denuded aortic rings precontracted with phenylephrine. Electrolysis of perfusion medium, released a vasodilator substance(s) from both. Vasodilatation was directly related to the current used and had t{sub 1/2} similar to that of EDRF released from EC columns by ADP. Release was attenuated by N-{omega}-nitro-L-arginine or sodium salicylate perfused through the cell column or aortic segments, or by 0.5% hemoglobin passed over the bioassay aortic rings. Finally, prompt transfer of 0.3-0.5 ml of medium, subjected to ES off-line, also relaxed pre-contracted aortic rings. Thus ES-FR can release EDRF-like substance(s) from both aortic EC and EC-denuded aortic smooth muscle. Since release was attenuated by salicylate, a specific trapping agent for hydroxyl radicals, the authors suggest that the latter is related to release of the vasodilator.

• OSTI ID: 5238563
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATTLE
DOMESTIC ANIMALS
DRUGS
ENDOTHELIUM
LUNGS
MAMMALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
RABBITS
RADICALS
RESPIRATORY SYSTEM
RUMINANTS
SECRETION
TISSUES
VASODILATORS
VERTEBRATES