Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.24.9265· OSTI ID:6464616

Ignarro, L J; Buga, G M; Wood, K S; Byrns, R E; Chaudhuri, G

The objective of this study was to determine whether nitric oxide (NO) is responsible for the vascular smooth muscle relaxation elicited by endothelium-derived relaxing factor (EDRF). EDRF is an unstable humoral substance released from artery and vein that mediates the action of endothelium-dependent vasodilators. NO is and unstable endothelium-independent vasodilator that is released from vasodilator drugs such as nitroprusside and glyceryl trinitrate. The authors have repeatedly observed that the actions of NO on vascular smooth muscle closely resemble those of EDRF. In the present study the vascular effects of EDRF released from perfused bovine intrapulmonary artery and vein were compared with the effects of NO delivered by superfusion over endothelium-denuded arterial and venous strips arranged in a cascade. EDRF was indistinguishable from NO in that both were labile inactivated by pyrogallol or superoxide anion, stabilized by superoxide dismutase, and inhibited by oxyhemoglobin or potassium. Both EDRF and NO produced comparable increases in cyclic GMP accumulation in artery and vein, and this cyclic GMP accumulation was inhibited by pyrogallol, oxyhemoglobin, potassium, and methylene blue. EDRF was identified chemically as NO, or a labile nitroso species, by two procedures. Thus, EDRF released from artery and vein possesses identical and biological and chemical properties as NO.

• Research Organization: Univ. of California, Los Angeles (USA)
• OSTI ID: 6464616
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:24
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NITRIC OXIDE
BIOLOGICAL EFFECTS
NITROSO COMPOUNDS
ARTERIES
CHEMICAL PROPERTIES
ENDOTHELIUM
HEMOGLOBIN
MUSCLES
RELAXATION
SUPEROXIDE DISMUTASE
VEINS
ANIMAL TISSUES
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
ENZYMES
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
TISSUES
560300* - Chemicals Metabolism & Toxicology