Detection of chromogranins A and B in endocrine tissues with radioactive and biotinylated oligonucleotide probes

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Surgical Pathology; (USA)

DOI:https://doi.org/10.1097/00000478-199001000-00004· OSTI ID:7244446

Lloyd, R V; Jin, L; Fields, K ^[1]

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1. Univ. of Michigan, Ann Arbor (USA)

We analyzed the distribution of chromogranins A and B in normal and neoplastic endocrine tissues with secretory granules using {sup 35}S-labeled and biotin-labeled oligonucleotide probes by in situ hybridization (ISH). Both radioactive and nonradioactive probes detected messenger RNAs (mRNAs) in frozen and paraffin tissue sections. Endocrine tissues with variable immunoreactivities for chromogranin A protein, such as small-cell lung carcinomas, neuroblastomas, insulinomas, and parathyroid adenomas, expressed the mRNA for chromogranins A and B in most cells. Some technical problems with the biotinylated probes included nonspecific nuclear staining and endogenous alkaline phosphatase, which was not completely abolished by levamisole pretreatment. A differential distribution of chromogranins A and B was seen in pituitary prolactinomas, which expressed abundant chromogranin B but not chromogranin A mRNAs, and in parathyroid adenomas, which expressed abundant chromogranin A but only small amounts of chromogranin B mRNAs. These results indicate that ISH can be used to detect chromogranins A and B in endocrine tissues with radioactive and biotinylated oligonucleotide probes and that the mRNAs for chromogranin A and B are demonstrable in some tumors even when the chromogranin proteins cannot be detected by immunohistochemistry.

• OSTI ID: 7244446
• Journal Information: American Journal of Surgical Pathology; (USA), Journal Name: American Journal of Surgical Pathology; (USA) Vol. 14:1; ISSN 0147-5185; ISSN AJSPD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOTIN
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
DNA HYBRIDIZATION
ENDOCRINE DISEASES
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
IMIDAZOLES
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOGENESIS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES
VITAMIN B GROUP
VITAMINS