In situ hybridization at the electron microscope level
Journal Article
·
· Scan. Microsc.; (United States)
OSTI ID:6477016
This tutorial paper will review radioactive and non-radioactive in situ hybridization (ISH) techniques at the electron microscope (EM) level; describe our efforts in comparison to those of other groups who recently have published in this field and discuss some potential future applications. Our contribution is the development of a non-radioactive, postembedding technique for the detection of transcripts on thin sections of Lowicryl K4M-embedded cells or tissues. Biotinylated probes were prepared by nick-translation. Signal detection was accomplished with anti-biotin antibody and protein A-gold complexes. Specific labeling was obtained over structures known to be the site of expression. Compared to EM autoradiography this technique offers advantages: rapid signal detection; superior morphological preservation and superior spatial resolution.
- Research Organization:
- Univ. of Vienna, Austria
- OSTI ID:
- 6477016
- Journal Information:
- Scan. Microsc.; (United States), Journal Name: Scan. Microsc.; (United States) Vol. 1; ISSN SCMIE
- Country of Publication:
- United States
- Language:
- English
Similar Records
Imaging of multiple mRNA targets using quantum dot based in situ hybridization and spectral deconvolution in clinical biopsies
SUPERSENSITIVE IN SITU HYBRIDIZATION BY TYRAMIDE SIGNAL AMPLIFICATION AND NANOGOLD SILVER STAINING: THE CONTRIBUTION OF AUTOMETALLOGRAPHY AND CATALYZED REPORTER DEPOSITION TO THE REJUVENATION OF IN SITU HYBRIDIZATION.
Deoxyribonucleic acid sequence mapping on metaphase chromosomes by immunoelectron microscopy
Journal Article
·
Fri Sep 22 00:00:00 EDT 2006
· Biochemical and Biophysical Research Communications
·
OSTI ID:20854475
SUPERSENSITIVE IN SITU HYBRIDIZATION BY TYRAMIDE SIGNAL AMPLIFICATION AND NANOGOLD SILVER STAINING: THE CONTRIBUTION OF AUTOMETALLOGRAPHY AND CATALYZED REPORTER DEPOSITION TO THE REJUVENATION OF IN SITU HYBRIDIZATION.
Book
·
Wed Apr 17 00:00:00 EDT 2002
·
OSTI ID:15006600
Deoxyribonucleic acid sequence mapping on metaphase chromosomes by immunoelectron microscopy
Journal Article
·
Sat Dec 31 23:00:00 EST 1988
· Scanning Microscopy, Supplement; (USA)
·
OSTI ID:7026969
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
COMPARATIVE EVALUATIONS
DNA
DOCUMENT TYPES
ELECTRON MICROSCOPY
HYBRIDIZATION
ISOTOPE APPLICATIONS
LABELLING
MICROSCOPY
MITOCHONDRIA
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
RESOLUTION
REVIEWS
SPATIAL RESOLUTION
TRACER TECHNIQUES
TRANSCRIPTION
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
COMPARATIVE EVALUATIONS
DNA
DOCUMENT TYPES
ELECTRON MICROSCOPY
HYBRIDIZATION
ISOTOPE APPLICATIONS
LABELLING
MICROSCOPY
MITOCHONDRIA
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
RESOLUTION
REVIEWS
SPATIAL RESOLUTION
TRACER TECHNIQUES
TRANSCRIPTION