Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin responsive DNA sequences upstream of the cytochrome P sub 1 -450 gene
Thesis/Dissertation
·
OSTI ID:7243151
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, dioxin) increases transcription of the cytochrome P{sub 1}-450 gene in a mouse hepatoma cell line. An intracellular TCDD receptor protein (the Ah receptor), and a cisacting enhancer element upstream of the cytochrome P{sub 1}-450 gene are required for TCDD-dependent transcription. In a clonal cell line stably transfected with a single copy of a plasmid containing the enhancer upstream of a viral promoter and the chloramphenicol acetyltransferase (CAT) gene, TCDD induces CAT transcription at the appropriate start site. Induction displays inducer specificity, TCDD sensitivity, and a time course of induction similar to those of the endogenous cytochrome P{sub 1}-450 gene. I conclude that the enhancer element and the receptor represent a general mechanism through which TCDD can mediate its pleiotropic effects.
- Research Organization:
- Stanford Univ., CA (USA)
- OSTI ID:
- 7243151
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DIOXIN
DISEASES
DNA SEQUENCING
ENZYMES
GENES
HEPATOMAS
HETEROCYCLIC COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MICE
MIXED-FUNCTION OXIDASES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PLASMIDS
PROTEINS
RECEPTORS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DIOXIN
DISEASES
DNA SEQUENCING
ENZYMES
GENES
HEPATOMAS
HETEROCYCLIC COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MICE
MIXED-FUNCTION OXIDASES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PLASMIDS
PROTEINS
RECEPTORS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES