Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on estrogenic responses
The competitive receptor binding affinities of thirteen 2-substituted-3,7,8-trichlorodibenzo-p-dioxins to hepatic cytosol from rat, mouse, guinea pig and hamster were determined using ({sup 3}H)-2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) as the radio-ligand. Significant species-dependent structural differences in the Ah receptor ligand binding site were observed and support the heterologous nature of the receptor protein. The interactions of 2,3,7,8-TCDD and estrogenic responses in the female rat and human breast cancer cells were also investigated. Cotreatment of 25-day-old female Long Evans rats with 20 or 80 ug/kg of 2,3,7,8-TCDD resulted in a dose-dependent decrease in both uterine and hepatic estrogen receptor (ER) levels. Moreover, these levels are decreased for at least ten days and appear to be related to the tissue persistence of 2,3,7,8-TCDD. In contrast, estradiol elevated uterine and hepatic ER levels and increased uterine wet weights. Cotreatment of the rats with 2,3,7,8-TCDD and estradiol resulted in hepatic and uterine ER levels which were comparable to those observed in the control rats; in addition, 2,3,7,8-TCDD also antagonized the effects of estradiol-induced uterine wet weights.
- Research Organization:
- Texas A and M Univ., College Station, TX (USA)
- OSTI ID:
- 7104260
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
BIOSYNTHESIS
DIOXIN
DOSE-RESPONSE RELATIONSHIPS
ESTROGENS
GENETIC VARIABILITY
GUINEA PIGS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
MAMMALS
MAN
MEMBRANE PROTEINS
MICE
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
STEROID HORMONES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES