Preferential localization and rate of loss of labeled alloantibody from rat tumors and skin transplants carrying the corresponding alloantigen

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Cancer Res.; (United States)

OSTI ID:7231966

Izzo, M J; Bale, W F

/sup 125/I-labeled rat histocompatibility antibody was prepared from sera of Buffalo rats immunized with a transplantable fibrosarcoma induced by methylcholanthrene in a Fischer 344 rat. After i.v. injection this antibody was shown to localize with high specificity in transplants of a second Fischer 344 tumor or Fischer 344 skin grafts onto immuno-suppressed Buffalo rats. These represent conditions such that only tumor or skin grafts carry the corresponding antigen. In skin transplants localization at 24 hr corresponded to 80 percent and in tumor to 30 percent of the injected dose in tissue equal to 1 percent of the rat weight. However, rapid loss of the /sup 125/I label from the target tissues indicated that degradation of antibody fixed to target cells was rapid and that the half-time of the intact antibody was less than 2 days.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7231966
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 36; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FIBROSARCOMAS
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
SARCOMAS
SKIN
TRANSPLANTS
VERTEBRATES