Localization in rat skin transplants of purified /sup 125/I-labeled xenogeneic histocompatibility antibody

Journal Article · Tue Feb 01 04:00:00 EST 1977 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-154-39633· OSTI ID:7306813

Izzo, M J; Contreras, M A; Bale, W F

It is shown that appropriate xenogeneic rabbit and horse antiserum, as well as allogeneic rat serum, can serve as sources from which /sup 125/I-labeled antibody to a strong histocompatibility antigen expressed on Fischer-344 strain rat cells can be prepared. After iv administration all three types of antibody would localize preferentially in F-344 skin grafts made 4 days earlier on Buffalo strain rats. After 24 hr, /sup 125/I localization ranged from 10 to 20 percent of the injected dose on F-344 skin grafts averaging 0.3 g weight on 150 to 190-g Buffalo rats. The horse serum used as an antibody source was a commercially available antirat-lymphocyte serum. Prior ip administration of two or three doses of 0.5 ml of this serum largely blocked uptake by F-344 skin grafts of both the /sup 125/I-labeled alloantibody and the /sup 125/I-labeled xenogeneic histocompatibility antibody prepared from this horse antiserum.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7306813
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 154:2; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HISTOLOGY
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
SKIN
VERTEBRATES