Survival of fetal rat pancreases frozen to -78 and -196/sup 0/
Transplantation of pancreases may have clinical utility in the treatment of diabetes, for it has been shown that chemically induced diabetes in rats can be reversed by the transplantation of greater than or equal to four syngeneic fetal pancreases. Allogeneic transplants produce serious immunological problems, but the problems could be ameliorated if tissue-typed organs could be stored in the frozen state. Unfortunately, nearly all attempts to freeze organs have failed. Nevertheless, recent developments in the physical-chemical analysis of freezing injury and its successful application to the freezing of mammalian embryos encouraged us to attempt the freezing of 16/sup 1///sub 2/- to 17/sup 1///sub 2/-day intact fetal pancreases. The analysis indicated that to achieve success pancreases would have to be cooled <1/sup 0//min and diluted extremely slowly after thawing. Experiments with embryos and red cells indicated that high survivals might require high concentrations of protective solutes and slow warming. These predictions were accurate. After freezing to -78 or -196/sup 0/ and thawing under optimal conditions, the fetal pancreases synthesized 80-100 percent as much protein as unfrozen controls and they yielded viable allografts. Optimal conditions included suspension in 2 M dimethylsulfoxide, freezing at 0.3/sup 0//min, and slow dilution to preclude osmotic shock.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- OSTI ID:
- 7227776
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 73:11
- Country of Publication:
- United States
- Language:
- English
Similar Records
Survival of frozen-thawed fetal rat pancreases as a function of the permeation of dimethylsulfoxide and glycerol, warming rate, and fetal age
Functional capacity and cryopreservation of fetal rat pancreas in streptozotocin-diabetes. [Effectiveness of transplantation of fetal pancreas for control of diabetes in adult rats]
Related Subjects
DMSO
BIOLOGICAL EFFECTS
FREEZING
PANCREAS
SURVIVAL TIME
TRANSPLANTS
THAWING
BIOSYNTHESIS
DIABETES MELLITUS
EMBRYOS
ERYTHROCYTES
FETUSES
IMMUNE REACTIONS
INJURIES
PROTEINS
RATS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RODENTS
SULFOXIDES
SYNTHESIS
VERTEBRATES
550500* - Metabolism