Survival of frozen-thawed fetal rat pancreases as a function of the permeation of dimethylsulfoxide and glycerol, warming rate, and fetal age
Journal Article
·
· Cryobiology; (United States)
Results of experiments concerned with the influence of cryobiological variables on the in vitro survival of frozen-thawed fetal rat pancreases (permeation of additive, warming rate, and dilution rate) and experiments concerned with the warming rate and dilution rate had no observable effect on the survival of 17-day fetal pancreases, but the permeation of additive was critical. Also critical was the age of the fetal pancreas, with the survival of 18- and 19-day fetal pancreases after freezing and thawing being much lower than that of 17-day fetal pancreases. (JMT)
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6706694
- Journal Information:
- Cryobiology; (United States), Vol. 18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DMSO
PERMEABILITY
FETUSES
AGE DEPENDENCE
GLYCEROL
PANCREAS
FREEZING
SURVIVAL TIME
THAWING
CRYOGENICS
IN VITRO
RATS
ALCOHOLS
ANIMALS
BODY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
HYDROXY COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RODENTS
SULFOXIDES
VERTEBRATES
551000* - Physiological Systems
DMSO
PERMEABILITY
FETUSES
AGE DEPENDENCE
GLYCEROL
PANCREAS
FREEZING
SURVIVAL TIME
THAWING
CRYOGENICS
IN VITRO
RATS
ALCOHOLS
ANIMALS
BODY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
HYDROXY COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RODENTS
SULFOXIDES
VERTEBRATES
551000* - Physiological Systems