Interaction of cooling rate, warming rate, glycerol concentration, and dilution procedure on the viability of frozen-thawed human granulocytes

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Cryobiology; (United States)

DOI:https://doi.org/10.1016/0011-2240(83)90070-6· OSTI ID:6054434

Frim, J; Mazur, P

The problem of preserving human granulocytes by freezing is addressed with emphasis on the fundamental cryobiology of the cell. The essential point if a cell is to survive freezing is surviving exposure to a sufficient concentration of protective additive to prevent slow-freezing injury. Glycerol was selected as the protective additive, and the permeability of the cell to glycerol was measured. The consequences of the osmotic volume changes associated with removing glycerol by dilution were investigated by using a fluorescent staining technique to measure cell viability both before and after the removal of glycerol. Fluorescent staining showed when the granulocytes were frozen, thawed, and diluted under optimal conditions, 50-60% survived. These relatively high survivals applied as long as the cells were held near 0/sup 0/C after thawing. However, relatively short exposure to 37/sup 0/C resulted in rapid killing. 33 references, 9 figures, 6 tables.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6054434
• Journal Information: Cryobiology; (United States), Vol. 20
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LEUKOCYTES
FREEZING
THAWING
CRYOBIOLOGY
EXPERIMENTAL DATA
GLYCEROL
LOW TEMPERATURE
MAN
PERMEABILITY
SURVIVAL TIME
ALCOHOLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
DATA
HYDROXY COMPOUNDS
INFORMATION
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PRIMATES
VERTEBRATES
560201* - Thermal Effects- Cells- (-1987)