Maturation of a single lambda phage particle from a dimeric circular lambda DNA
Journal Article
·
· Virology; (United States)
An Escherichia coli phage lambda tandem dilysogen containing prophages defective in phage DNA replication and genetic recombination and separated by a defective attachment site was used to study the maturation process of phage lambda. In such a lysogen, normal prophage excision proceeds very slowly compared to maturational cutting. By a simple variation of the induction protocol, normal excision can be made to precede maturational cutting, resulting in the excision of a dimeric circular lambda DNA. Single-burst analysis of cells carried through this protocol shows that only one monomer unit of such a dimer can be packaged.
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 7227760
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 74:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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