Instrumental and theoretical development of capillary electrophoresis and its application to biological systems
Essential aspects of capillary electrophoresis (CE) are outlined. By integrating the electrophoretic current during electromigration, a migration index and an adjusted migration index are used to compare results obtained under changing conditions (constant and gradient fields, capillary lengths, inner diameters, {zeta}-potentials). Except for inner diameter cases, the indices are superior to all other indicators, and the qualitative precision of migration data is improved. Integration of the current during injection raises the quantitative precision of manual electrokinetic injection to the levels attained with hydrodynamic injection. A model based on field-amplified CE is proposed to describe the electrokinetic injection process involving samples with distinct conductivities and an equation is developed to allow normalization of peak areas at the 1--5 % level without the need for quantitative internal standardization. Experiments reveal that the model is valid when electromigration is the predominant mode of migration during injection. Design and performance of a rugged, compact, user-friendly and easily constructed laser-induced fluorescence (LIF) detector for CE are presented.
- Research Organization:
- Ames Lab., IA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 7224364
- Report Number(s):
- IS-T-1629; ON: DE93001220
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ELECTROPHORESIS
CAPILLARY FLOW
KINETICS
OLIGONUCLEOTIDES
PEPTIDES
VACCINES
DETECTION
ERYTHROCYTES
FLUORIMETERS
MEASURE THEORY
MICELLAR SYSTEMS
MIGRATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
FLUID FLOW
MATERIALS
MATHEMATICS
MEASURING INSTRUMENTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
550200* - Biochemistry
400102 - Chemical & Spectral Procedures
400105 - Separation Procedures