Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis

Journal Article · Mon Jun 01 00:00:00 EDT 1992 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac00035a007· OSTI ID:478546

Lee, T T; Yeung, E S ^[1]

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1. Iowa State Univ., Ames, IA (United States)

The quantitative precision in capillary zone electrophoresis (CZE) with electrokinetic injection can be improved by proper correction with the integrated current during injection. By monitoring the electrophoretic current during separation, one can also correct for temporal fluctuations in the analyte migration velocity. A model based on field-amplified capillary electrophoresis is used to describe the electrokinetic injection process involving sample solutions with distinct conductivities, and an equation is developed to allow normalization of peak areas at the 0-5% level without the need of quantitative internal standardization. 28 refs., 2 figs., 2 tabs.

• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 478546
• Journal Information: Analytical Chemistry (Washington), Vol. 64, Issue 11; Other Information: PBD: 1 Jun 1992
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
ELECTROPHORESIS
MATERIALS
SAMPLING
STANDARDIZATION
MATHEMATICAL MODELS