Isolation, characterization, and regional mapping of microclones from a human chromosome 21 microdissection library

Journal Article · Sat Aug 01 00:00:00 EDT 1992 · American Journal of Human Genetics; (United States)

OSTI ID:7224334

Yu, J; Hartz, J; Xu, Yisheng; Gemmill, R M; Patterson, D; Kao, Faten ^[1]; Gemmill, R M; Patterson, D; Kao, Fa-Ten ^[2]; Korenberg, J R ^[3]

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1. Eleanor Roosevelt Inst., Denver, CO (United States)
2. Univ. of Colorado Health Sciences Center, Denver, CO (United States)
3. Univ. of California, Los Angeles (United States)

Thirty-four unique-sequence microclones were isolated from a previously described microdissection library of human chromosome 21 and were regionally mapped using a cell hybrid mapping panel which consists of six cell hybrids and divides chromosome 21 into eight regions. The mapping results showed that the microclones were unevenly distributed along chromosome 21, with the majority of microclones located in the distal half portion of the long arm, between 21q21.3 and 21qter. The number of unique-sequence clones began to decrease significantly from 21q21.2 to centromere and extending to the short arm. This finding is consistent with those reported in other chromosome 21 libraries. Thus, it may be inferred that the proximal portion of the long arm of chromosome 21 contains higher proportions of repetitive sequences, rather than unique sequences of genes. The microclones were also characterized for insert size and were used to identify the corresponding genomic fragments generated by HindIII. In addition, the authors demonstrated that the microclones with short inserts can be efficiently used to identify YAC (yeast artificial chromosome) clones with large inserts, for increased genomic coverage for high-resolution physical mapping. They also used 200 unique-sequence microclones to screen a human liver cDNA library and identified two cDNA clones which were regionally assigned to the 21q21.3-q22.1 region. Thus, generation of unique-sequence microclones from chromosome 21 appears to be useful to isolate and regionally map many cDNA clones, among which will be candidate genes for important diseases on chromosome 21, including Down syndrome, Alzheimer disease, amyotrophic lateral sclerosis, and one form of epilepsy.

• OSTI ID: 7224334
• Journal Information: American Journal of Human Genetics; (United States), Vol. 51:2; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEREDITARY DISEASES
BIOLOGICAL MARKERS
HUMAN CHROMOSOME 21
GENETIC MAPPING
DNA SEQUENCING
DNA-CLONING
GENES
LIVER
MAN
MOLECULAR BIOLOGY
MUTANTS
RECOMBINANT DNA
YEASTS
ANIMALS
BODY
CHROMOSOMES
CLONING
DIGESTIVE SYSTEM
DISEASES
DNA
DNA HYBRIDIZATION
EUMYCOTA
FUNGI
GLANDS
HUMAN CHROMOSOMES
HYBRIDIZATION
MAMMALS
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLANTS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550400* - Genetics